Journal: STAR Protocols

Article Title: Protocol for generating human craniofacial cartilage organoids from stem-cell-derived neural crest cells

doi: 10.1016/j.xpro.2024.103532

Figure Lengend Snippet:

Article Snippet: Use EasySep Release Human PSC-derived Neural Crest Cell Positive Selection Kit, Stem cell Technologies, Cat# 100–047 to sort the cells based on cell surface CD271 (NGFR) marker using magnetic beads as described in the following attached kit manufacturer’s protocol: https://cdn.stemcell.com/media/files/pis/10000007951-PIS_00.pdf . j.

Techniques: Recombinant, Knock-Out, Membrane, Selection

Journal: International Journal of Molecular Sciences

Article Title: Blocking the PCNA/NKp44 Checkpoint to Stimulate NK Cell Responses to Multiple Myeloma

doi: 10.3390/ijms23094717

Figure Lengend Snippet: Blocking the membranal-PCNA on MM cell lines enhances IFN-γ secretion and degranulation of NK cells. ( A ) Primary NK cells from a healthy donor were incubated with U266 cell lines and mAb 14-25-9 or mIgG1 for 18 h using different ratios of effector:target (1:1 and 1:3). IFN-γ was measured by ELISA. ( B ) Primary NK cells from a healthy donor were incubated either with RPMI 8226 or U266 cell lines and mAb 14-25-9 or mIgG1 for 5 h. Surface CD107a was measured by flow cytometry, to detect NK cells that had degranulated. Mouse IgG1 acts as a background control (red bars) in comparison to mAb 14-25-9 (blue bars). Dead cells were discriminated against using 7-AAD. Data is shown from three independent replicates and the experiment was repeated three times. Two-way ANOVA test showed p ** < 0.01 and p *** < 0.001. Statistical analysis and design were performed using Prism GraphPad v8 (California).

Article Snippet: Cells were isolated using a human negative selection-based NK cell isolation kit (EasySep-19615, STEMCELL).

Techniques: Blocking Assay, Incubation, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Control, Comparison

Journal: International Journal of Molecular Sciences

Article Title: Blocking the PCNA/NKp44 Checkpoint to Stimulate NK Cell Responses to Multiple Myeloma

doi: 10.3390/ijms23094717

Figure Lengend Snippet: Blocking the membranal-PCNA on primary BM mononuclear cells from MM patients enhanced the activation of NK cells. ( A ) NKp44 isoform 1-transduced NK92 cells (NK92-44-1) were incubated either with Pat#MM2 or Pat#MM7 primary cells for 18 h. ( B ) Primary NK cells from a healthy donor were incubated either with Pat#MM2 or Pat#MM7 primary cells for 18 h. ( C ) Primary NK cells from another unrelated healthy donor were incubated either with Pat#MM3.1 or Pat#MM4.1 primary cells for 18 h. Mouse IgG1 was used as a background control (red bars) for mAb 14-25-9 (blue bars). IFN-γ was measured by ELISA. Data is shown of three technical replicates. Two-way ANOVA test showed p * < 0.05, p ** < 0.01, and p *** < 0.001. Statistical analysis and design were performed using Prism GraphPad v8.

Article Snippet: Cells were isolated using a human negative selection-based NK cell isolation kit (EasySep-19615, STEMCELL).

Techniques: Blocking Assay, Activation Assay, Incubation, Control, Enzyme-linked Immunosorbent Assay